eagle-i The Ohio State UniversityThe Ohio State University


eagle-i ID


Resource Type

  1. Service


  1. Fee for service
  2. Resource Description
    The Facility can perform Genotyping by two methods: fragment analysis and high resolution melt analysis. The facility has a 3730 DNA Analyzer (Applied Biosystems, Inc.) that can analyze DNA fragments labeled with a fluorescent dye to determine the size of the fragment with a resolution of one base. With 48 capillaries the instrument can analyze up to 48 samples simultaneously as well as accommodate 96-, or 384-well plates. The 3730 DNA Analyzer has the ability to multiplex within one capillary by the use of 5 different fluorescent dyes, and/or differing fragment size. The facility can perform such techniques as allelic determination, amplified length polymorphism analysis (AFLP), microsatellite analysis (STR and SSR), and single nucleotide polymorphism analysis (SNapshot). The BioRad CFX96 real-time system can be used a specialized fluorometric based plate reader that can detect most dyes for applications such as high resolution melt analysis, single nucleotide polymorphism detection and measuring protein melting.
  3. Service Fee URL
  4. Service Fee URL
  5. Additional Name
    Amplified Fragment Length Polymorphism (AFLP)
  6. Additional Name
    High Resolution Melt Analysis (HRMA)
  7. Additional Name
    Human DNA Fingerprinting (Human ID)
  8. Additional Name
    Microsatellite analysis (MS)
  9. Additional Name
    Single Nucleotide Polymorphism analysis (SNaP Shot)
  10. Related Resource
    AppliedBiosystems 3730 DNA Analyzer
  11. Related Resource
    BioRad CFX96 real-time System
  12. Access Restriction(s)
    Available to Everyone
  13. Service Provided by
    Plant-Microbe Genomics Facility
  14. Website(s)
Provenance Metadata About This Resource Record

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016