eagle-i The Ohio State UniversityThe Ohio State University

Promoter Characterization

eagle-i ID

http://eagle-i.rf.ohio-state.edu/i/0000014c-c2a3-f210-ef51-a5b180000000

Resource Type

  1. Support service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    Two traditional methods, DNA protection assay (DNA Footprint) and primer extension, that are used to determine promoter binding sites and transcription start sites respectively can be done at the Facility. Instead of using radioactivity and a porlyacrylamide gel the DNA is labeled with a fluorescent dye and the resulting fragments from the methods above can be analyzed with the automated capillary electrophoresis instrument at the Facility, i.e. a 3730 DNA Analyzer. In addition, non-radioactive gel shift assays are also commonly performed along with DNA Footprinting which the Facility also provides as a service.
  3. Service Fee URL
    http://pmgf.osu.edu/fees/promoter
  4. Additional Name
    DNA Foot Print Analysis (DFACE)
  5. Additional Name
    Electropherotic Mobility Shift Assay (EMSA)
  6. Additional Name
    mRNA Start Site Analysis (MSACE)
  7. Related Resource
    AppliedBiosystems 3730 DNA Analyzer
  8. Related Resource
    BioRad VersaDoc 4000MP Imaging System
  9. Access Restriction(s)
    Available to Everyone
  10. Service Provided by
    Plant-Microbe Genomics Facility
  11. Website(s)
    http://pmgf.osu.edu/services/promoter
 
RDFRDF
 
Provenance Metadata About This Resource Record

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016