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Multiphoton

eagle-i ID

http://eagle-i.rf.ohio-state.edu/i/00000150-f210-8137-4a4c-b9f280000000

Resource Type

  1. Two-photon confocal microscope

Properties

  1. Resource Description
    The Olympus FV1000 MPE Multiphoton Laser Scanning Confocal is equipped with a DeepSee MaiTai titanium -sapphire laser, a 25x1.05 N.A. 2mm working distance water immersion objective lens, four non-descanned detectors and a forward, second harmonic generation (SHG) detector. This microscope enables researchers to image deep into fixed or living tissues since the infrared light can penetrate up to 1 millimeter into the sample. Several support instruments are available for intravital, live cell or live tissue imaging including a temperature controlled imaging chamber and a rodent oximeter. A vibratome is available for thick sectioning of fixed tissues. This system also functions as a standard confocal microscope system with 3 fluorescence detectors and a transmitted detector for DIC imaging. A motorized stage is available for capturing tiled images or tiled image stacks. Additional water immersion objective lenses are available for use with the Multiphoton including a 40X lens and a 100X lens. Dry and oil objective lenses can also be used on this system.
  2. Additional Name
    Olympus FV1000 MPE Multiphoton Laser Scanning Confocal
  3. Contact
    CMIF Staff
  4. Website(s)
    http://www.cmif.osu.edu/Microscopy-Multiphoton
  5. Location
    Campus Microscopy and Imaging Facility
 
RDFRDF
 
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