Consultation (Design and Implementation of Proteomics Studies)

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   PSR personnel provide assistance to individual investigators with the design and implementation of proteomics studies, in particular those involving mass spectrometry. The benefits of mass spectrometry-based proteomics methods are numerous, including routine femtomole range sensitivity, rapid analysis speed and, most importantly, the ability to precisely and accurately determine protein identity and characterize modifications. Quantitative proteomics can also track the entire differential output of proteins and protein complexes by systems under disease, drug or environmental challenge. The PSR provides a unique shared resource that is both cutting-edge and affordable for the entire OSUCCC membership. Equally important is the desire of PSR faculty and staff to educate the OSUCCC membership about the potential applications for mass spectrometry and proteomics in cancer research and assist members in acquiring the most advanced equipment coupled with the knowledge to operate it. A proteomic project is never a “one size fits all” experiment, so each project begins with a meeting involving key personnel on the project. At the meeting, the researcher describes the goals of the project and the proteomics team (Drs. Arpad Somogyi, Liwen Zhang, Sophie Harvey, Andrew Reed and Miranda Gardner) will recommend the specific experimental plan and discuss the details of the sample preparation the researcher will need to follow for a successful experiment. Possible pitfalls and alternative solutions are also discussed. Following the meeting, the researcher prepares a small trial sample set used to generate preliminary data, to perform any necessary method development and to determine the likely success or failure of the project. If the trial sample is successful, the researcher prepares the full sample set required for the experiment. If problems are encountered with the trial sample, the PSR can often determine the particular pitfall in this sample and assist the researcher to solve the problem quickly. This process has been very successful in supporting the PSR’s ability to generate high-quality, publishable data in a timely manner. An added goal of the PSR – to develop even more advanced methods for sample pre-fractionation prior to mass spectrometric analysis – is also highly beneficial to the membership. Gel-free electrophoresis equipment is available for protein separation, especially for less soluble hydrophobic membrane proteins, post-translationally modified proteins and proteins that express at a low level. These resources also provide analyses of complex mixtures of proteins independent of 2D gel electrophoretic separations.

3. Service Fee URL

   https://www.ccic.osu.edu/MSP-Rates

4. Contact

   Liwen Zhang

5. Service Provided by

   The CCC Proteomics Shared Resource (PSR)

6. Website(s)

   https://cancer.osu.edu/for-cancer-researchers/resources-for-cancer-researchers/shared-resources/proteomics/services-and-fees

7. Website(s)

   https://www.ccic.osu.edu/msp

8. Part of Collection

   The Ohio State University Comprehensive Cancer Center Collection