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Small Animal Imaging Core

Director: Kimerly Powell

Summary:

The Small Animal Imaging Core (SAIC) is a small-animal imaging facility available to all investigators and other academic and commercial institutions.

The SAIC houses high-resolution imaging equipment and offers assistance from professionals who are experts in operating each imaging modality, small animal handling, and analytical software support for quantitative image analysis.

Affiliations:

People:

Resources:

Instruments

  • BioSpec 94/30 MRI System ( MRI Scanner )

    The BioSpec 94/30USR (Bruker BioSpin Co.) Imaging System is a 9.4T horizontal bore magnet that operates at 400 MHz and runs ParaVision™ 6.0 software. This microMRI system is designed for high-resolution MR spectroscopy and imaging of in vivo or ex vivo specimens.

    The 30 cm diameter bore and extra-long table offers imaging capabilities of 1H, 13C, 19F, 23Na, 31P on mice, rats, small rabbits and ex vivo specimens. The BioSpec also includes a BioTrig monitoring unit and in vivo cardiac gating (SAII and IntraGate).

    MRI applications include:

    Skeletal and soft tissue abnormalities
    DCE-MRI for tumor/tissue vascularization and permeability
    In vivo spectroscopy to measure metabolites (e.g. 31P)
    In vivo localized spectroscopy to measure metabolites (1H)
    Cardiac imaging for assessing cardiac function, ischemia and infarcts (Cardio Package)
    T1, T2 and Diffusion maps
    Diffusion Tensor Imaging
    Others

  • CT and Therapeutic X-ray (SARRP) - (XSTRAHL Inc.) ( X-ray source )

    The SARRP system (XSTRAHL Inc.) delivers clinically relevant radiation protocols under preclinical conditions. The SARRP system can deliver radiation dose locally with very precise collimators from 0.5 mm small focal spot to 10*10 mm window, variable collimators up to 40*80 mm or with open field (typical 15*15 cm, bigger field is possible per application).

    The SARRP system can be used for:

    Local tumor treatment with minimalized dose to whole body or individual organs
    To sensitize lesion to different treatment
    Total Body Irradiation (TBI) for e.g. bone marrow or autologous stem cell transplantation
    Studying skin wounds
    Others

  • EchoMRI (EchoMRI LLC) Analyzer ( Body composition analyzer )

    Body Composition Analyzer
    EchoMRI (EchoMRI LLC) Analyzer delivers precise body composition measurements of fat, lean tissue, free water and total water masses in live animals (up to 100g), organ or biopsy (up to 300µg). Scanning takes 0.5-3.2 minutes. The live animals need no anesthesia and no special preparation before measurement.

    EchoMRI system can be used for cancer, cardiac, toxicology, diabetic, nutritional studies and others.

  • Gammacell® 40 Exactor Low Dose-Rate Research Irradiator With 2 Caesium-137 Sources ( Radiation measurement device )

    Gammacell® 40 Exactor (Best Theratronics Ltd.) Low Dose-Rate Research Irradiator With 2 Caesium-137 Sources

    Unit is ready for immediate cell experiment applications with central dose rate ~1.1 Gy/min and dose uniformity of ± 7% over a 260 mm (10.14 in.) diameter and a 100 mm (3.9 in.) height.

    The Gammacell system can be used for:

    Cell irradiation to induce apoptosis, study radiation effects, induce gene mutations, etc.

    Small animals Total Body Irradiation (TBI) for e.g. bone marrow or autologous stem cell transplantation. Note for users: Please parafilm each dish and use your own properly sized secondary containment. Pie cages are not provided.

  • IVIS Lumina II (PerkinElmer Inc.) Bioluminescent Optical Imaging System ( In vivo fluorescence imaging system )

    The CaliperTM IVIS Lumina II Bioluminescent Optical Imaging System is suitable for 2D in vivo and ex vivo bioluminescence (BLI) and fluorescence (FL) imaging. The system is equipped with four standard emission filters ICG, Cy5.5, DsRed, GFP (810-875, 695-770, 575-650, 515-575 nm) for fluorescence imaging.

    IVIS Lumina II applications include:

    1) A powerful labeling technique through gene expression results in production of luciferase
    2) Provides analysis tools for quantification
    3) Provides in vivo fluorescence imaging (preferable near-infrared)
    4) Provides ex vivo and in vitro BLI and FL imaging

  • RS-2000 Biological Irradiator for Small Animals and Cells ( Radiation measurement device )

    RS-2000 (Radsource, Inc.) for cells or small-animal irradiation with dose rate ~ 1.2 Gy/min (animal), 5-10 Gy/min (cells). Patented RAD+ technology allows for a dose uniformity of 94%. Allows to irradiate multiple enclosures during one cycle.

    The irradiator can be used for:
    Cell irradiation
    Total Body Irradiation (TBI) or partial body irradiation

  • SkyScan 1276 micro-CT (Bruker BioSpin Co.) ( Computed tomography scanner )

    Computed Tomography (micro-CT)

    The SkyScan 1276 (Bruker BioSpin Co.) is a high-performance, standalone, fast, desktop in vivo microCT with continuously variable magnification for scanning small laboratory animals (mice and rats) and biological samples. It has an unrivalled combination of high resolution (2.8µm), big image size as well as software allowing to acquire data with breathing and ECG gating. It is capable of reconstructing 3D high resolution images throughout the animal body that can be used for quantitative morphometric measurements. The software also allows to perform quantitative bone density measurement.The SkyScan system can be used for:

    Whole body mouse imaging
    Lung imaging in mice and rats (e.g. tumor monitoring)
    Bone imaging and bone density measurements
    Cardiovascular imaging
    Vascularization imaging e.g. brain, tumors, ischemic tissue
    Post contrast imaging for miscellaneous applications

  • Vevo 2100 High-Frequency Ultrasound System ( In vivo imaging system )

    The Vevo 2100 High-Frequency Ultrasound System is capable of 2D and 3D in vivo imaging in small animals and has a 30-micron spatial resolution and frame rates of up to 1000 fps. The system includes an 18, 24, 38 and 55 MHz transducer, a needle-guided injection apparatus, and is capable of color, pulsed wave and power Doppler, 3D imaging and advanced image analysis.

    The Vevo can be used for:

    1) Image-guided injections
    2) Evaluating developmental biology – mouse and rat embryos
    3) Calculating tumor size and blood flow
    4) Quantitative evaluation of cardiac physiological function and development

Resource Collection

  • The Ohio State University Comprehensive Cancer Center Collection ( Resource Collection )

    The OSUCCC – James Shared Resources and core facilities provide specialized services to cancer researchers. They offer cost-effective, state-of-the-art technology and instrumentation; expert guidance and training; and clinical, administrative and technical support.

Services


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Last updated: 2021-01-05T12:04:17.265-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016