The Flow Cytometry Shared Resource (FCSR) provides state-of-the-art flow cytometry analysis and sorting of cell populations using selected cellular markers. Flow cytometry is a critical technology for cancer research, and the ACSR is used extensively by all scientific programs in the OSUCCC – James and by the broader Ohio State research community.
Researchers can learn more about the use and application of flow cytometry in several ways, including scientific seminars, marketing at cancer-specific meetings, instrumentation overviews and application workshops. Additionally, the Mid-Ohio Cytometry Associates “users’ group” meets regularly to discuss relevant cytometry issues and to generate educational opportunities.
Amnis Imagestream X Mark II - Imaging Flow Cytometer. 4 laser (405, 488, 561, 642) 10 fluorescent channels
Becton Dickinson FACS Aria II - 4 laser (355, 488, 561, 633), 13 fluorescent detectors
Becton Dickinson FACS Aria IIu - 2 laser (488, 633), 7 fluorescent detectors.
Becton Dickinson LSR Fortessa - 5 laser (355, 405, 488, 561, 642), 18 fluorescent detectors.
Becton Dickinson LSR II - 3 laser (405, 488, 642), 13 fluorescent detector
NanoSight NS300 – allows rapid analysis of size and concentration of nanoparticles from 0.01 – 1 um in diameter. It has a 532 nm laser for detection of a single color of fluorescence.
The OSUCCC – James Shared Resources and core facilities provide specialized services to cancer researchers. They offer cost-effective, state-of-the-art technology and instrumentation; expert guidance and training; and clinical, administrative and technical support.
Post acquisition data analysis, help with creating figures and plots for publication, some training in use of data analysis software.
Staff help with recording and/or sorting of samples.
Training on use of equipment, includes software and basics of flow cytometry
Use of instrumentation without staff assistance, training required
Site licensed flow cytometry data analysis software.