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Flow Cytometry Shared Resource

Summary:

The Flow Cytometry Shared Resource (FCSR) provides state-of-the-art flow cytometry analysis and sorting of cell populations using selected cellular markers. Flow cytometry is a critical technology for cancer research, and the ACSR is used extensively by all scientific programs in the OSUCCC – James and by the broader Ohio State research community.

Researchers can learn more about the use and application of flow cytometry in several ways, including scientific seminars, marketing at cancer-specific meetings, instrumentation overviews and application workshops. Additionally, the Mid-Ohio Cytometry Associates “users’ group” meets regularly to discuss relevant cytometry issues and to generate educational opportunities.

Affiliations:

People:

    Resources:

    Instruments

    • Amnis Imagestream X Mark II ( Flow cytometer )

      Amnis Imagestream X Mark II - Imaging Flow Cytometer. 4 laser (405, 488, 561, 642) 10 fluorescent channels

    • BD FACS Aria II ( Flow cytometer sorter )

      Becton Dickinson FACS Aria II - 4 laser (355, 488, 561, 633), 13 fluorescent detectors

    • BD FACS Aria IIu ( Flow cytometer sorter )

      Becton Dickinson FACS Aria IIu - 2 laser (488, 633), 7 fluorescent detectors.

    • BD LSR Fortessa ( Flow cytometer )

      Becton Dickinson LSR Fortessa - 5 laser (355, 405, 488, 561, 642), 18 fluorescent detectors.

    • BD LSR II ( Flow cytometer )

      Becton Dickinson LSR II - 3 laser (405, 488, 642), 13 fluorescent detector

    • NanoSight NS300 ( Nanoparticle size analyzer )

      NanoSight NS300 – allows rapid analysis of size and concentration of nanoparticles from 0.01 – 1 um in diameter. It has a 532 nm laser for detection of a single color of fluorescence.

    Resource Collection

    • The Ohio State University Comprehensive Cancer Center Collection ( Resource Collection )

      The OSUCCC – James Shared Resources and core facilities provide specialized services to cancer researchers. They offer cost-effective, state-of-the-art technology and instrumentation; expert guidance and training; and clinical, administrative and technical support.

    Services

    Software


    Web Links:

    Last updated: 2021-03-22T09:21:15.199-04:00

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016