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Campus Microscopy and Imaging Facility / Microscopy Shared Resource (CMIF/MSR)

Director: Paul Stoodley

Summary:

The Campus Microscopy and Imaging Facility (CMIF) serves University faculty staff and students as well as researchers outside Ohio State. The CMIF offers a full range of light, confocal, live-cell, super-resolution, and electron microscopes. Sample preparation equipment is available. Expert support and technical assistance enables users to collect and analyze high quality images.

This facility also functions as the Microscopy Shared Resource (MSR) which facilitates cancer research by providing an accessible centrally-organized resource with technical support for confocal, light, live cell microscopy, super-resolution microscopy and transmission electron microscopy. Highly qualified faculty and staff provide training and advice for sample preparation, processing fresh tissues, sectioning epoxy-embedded samples, basic microscopy, and advanced training in live animal multiphoton microscopy. The MSR, a part of the larger Campus Microscopy and Imaging Facility under the OSU Office of Research, is managed by the OSUCCC. The MSR is located within OSUCCC space in the Biomedical Research Tower. The ability to offer competitive pricing and exceptional service can be largely attributed to the generous support of outstanding institutional resources, including partnerships with the OSUCCC, the Office of Research, OSU Colleges and Institutes, and state and federal grants. The Microscopy Shared Resource (MSR) gives researchers access to state-of-the-art microscopes and to services ranging from standard light and electron microscopy to leading-edge, live-animal, multiphoton microscopy. MSR experts support high-level cancer research with the latest microscopy techniques.

Affiliations:

People:

Resources:

Instruments

  • Critical Point Dryer ( Automatic Critical Point Dryer) ( Critical point dryer )

    Tousimis - Autosamdri-931 Automatic Critical Point Drier for drying biological and material samples for Scanning Electron Microscopy.

  • Microscope - Confocal (Live-Cell Laser Scanning Confocal Microscope) ( Inverted microscope )

    Nikon A1R Live-Cell Confocal Imaging system with auto-focus and Resonant Scanner for good resolution fast confocal imaging of living processes for
    real time imaging. Multi-Laser line and DIC imaging capability.

  • Microscope - Confocal Microscope (Laser Scanning Confocal Microscope) ( Laser scanning confocal microscope )

    Olympus FV3000 Laser Scanning Confocal Microscope with five lasers from 405 to 752 nm, high sensitivity detectors and Resonant Scanning Capability
    for high speed scanning.

  • Microscope - Confocal Microscope (Multi Photon Microscope). ( Confocal microscope )

    Olympus Fv1000 Multi-Photon Microscope provides both live-cell confocal and live-cell Multi-Photon imaging. Multi-Photon Imaging causes significantly less tissue damage in living cells and can penetrate much deeper into tissues than traditional confocal imaging.

  • Microscope - Super Resolution Microscope (SIM, TIRF, STORM) ( super-resolution microscope )

    Nikon Super-Resolution Microscope with SIM,TIRF and STORM capabilities for extremely high resolution imaging with living and fixed biological samples as well as material samples.

  • Microscope- Electron Microscope (Transmission Electron Microscope) ( Transmission electron microscope )

    FEI Tecnai G2 Biotwin TEM provides excellent contrast and imaging of biological sections and negatively stained samples.

  • Microscope- Widefield (Fluorescence / Bright-Field Microscope) ( Fluorescence microscope )

    Zeiss Axioskop Light Microscope with Digital camera for Widefield Imaging of Fluorescent and Bright-field samples. Objectives from 4x air to 100x oil and
    phase-contrast imaging capability.

  • Microtome - Cryostat (Cryotome) ( Microtome cryostat )

    Microtome - Cryostat (Cryotome) Thermo-Fisher Cryotome E Cryostat for sectioning frozen biological and material samples for Widefield Light and Confocal Microscopy.

  • Microtome- Cryo Ultramicrotome ( Microtome cryostat )

    Leica EM UC6 Ultramicrotome for Ambient sectioning and with EM FC6 for cryo-sectioning for Transmission Electron Microscopy (TEM)

  • Multiphoton ( Two-photon confocal microscope )

    The Olympus FV1000 MPE Multiphoton Laser Scanning Confocal is equipped with a DeepSee MaiTai titanium -sapphire laser, a 25x1.05 N.A. 2mm working distance water immersion objective lens, four non-descanned detectors and a forward, second harmonic generation (SHG) detector. This microscope enables researchers to image deep into fixed or living tissues since the infrared light can penetrate up to 1 millimeter into the sample. Several support instruments are available for intravital, live cell or live tissue imaging including a temperature controlled imaging chamber and a rodent oximeter. A vibratome is available for thick sectioning of fixed tissues.

    This system also functions as a standard confocal microscope system with 3 fluorescence detectors and a transmitted detector for DIC imaging. A motorized stage is available for capturing tiled images or tiled image stacks. Additional water immersion objective lenses are available for use with the Multiphoton including a 40X lens and a 100X lens. Dry and oil objective lenses can also be used on this system.

  • Sputter Coater- Automatic Sputter Coater (High Resolution Coater) ( Automatic sputter coater )

    EMS High resolution sputter coater 150TS for coating biological and material non-conductive samples for Scanning Electron Microscopy (SEM)

Resource Collection

Services

  • Consulting on Experimental Design ( Training service )

    Experimental design assistance , controls etc.

  • Fixative Preparation ( Material processing service )

    Preparation of EM fixative for TEM and SEM samples.

  • Image Analysis Assistance ( Support service )

    Provide training and support to users wanting image analysis. High quality Imaris image-analysis software and other analysis software are available on the Image Analysis Workstation.

  • Paid Service Imaging ( Support service )

    Users who do not wish to be trained may pay staff members to run the microscopes and equipment for them.

  • Technical Assistance with Equipment ( Training service )

    Training and support for users on microscopes and equipment.

  • TEM - Thin Sectioning ( Material processing service )

    TEM blocks thin sectioned and stained ready for TEM imaging.

  • TEM -Thick sectioning ( Material processing service )

    TEM blocks thick sectioned and stained ready for Light Microscope examination. Blocks may then be trimmed down for Thin-sectioning.

  • TEM Cryo-Sectioning ( Material processing service )

    TEM cryo-sectioning of biological and material samples for subsequent TEM or cryo-TEM imaging.

Software

  • Imaris Image Analysis Software ( Software )

    Imaris is a state-of-the-art Image analysis Software program on the Image-Analysis Workstation. Training by appointment is available.


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Last updated: 2021-03-22T09:16:12.903-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016