The Ohio State Human Tissue Resource Network (HTRN) facilitates research biospecimen access for funded, IRB approved or reviewed for non-human subject status (basic and translational investigators). HTRN is the organizational umbrella for National Cancer Institute (NCI) funded programs to advance bench to bedside research. Investigator biospecimen access is for investigators from The Ohio State University, as well as for basic and translational researchers throughout the United States and Canada. These NCI funded groups join to improve resource utilization and thereby reduce the researcher fees for samples. Services include biospecimen and biofluid procurement by the Department of Pathology Tissue Procurement Services and processing, transient storage, biobanking and virtual microscopy with and without digital image analysis as requested.
The HTRN biorepository is comprised of samples for the ALLIANCE for Clinical Trials and Oncology, the Epidemiology of Burkitt Lymphoma in East-African Children and Minors (EMBLEM) and special projects from principle investigators within OSUWMC.
The RTPS procures and distributes fluids and benign, normal, malignant, and diseased tissues to research investigators.
Tissues and fluids are collected prospectively, as per the investigator’s protocol, or through patient consent for OSU protocol specific clinical trials and banking and can be provided to the researcher in various preparations including fresh, frozen, frozen OCT, frozen RNA Later, formalin-fixed paraffin-embedded tissue, unstained frozen section slide, unstained paraffin section slide, H&E-stained paraffin section slide and frozen serum/plasma and urine.
Biospecimen types available include:
5) Biofluids (urine, serum, plasma, buffy coat)
Anatomic sites include but are not limited to the following:
1) Breast, Uterus & Ovary
2) Colon, Stomach, and Esophagus
3) Kidney, Bladder & Prostate
4) Liver, Pancreas, & Spleen
5) Lung, Pharynx, & Oral Cavity
6) Brain, Spinal Cord, & Peripheral Nerves
7) Muscle, Skin & Soft Tissue
The Tissue Archive Service (TAS) was established within the Department of Pathology at THE Ohio State University Wexner Medical Center in June 1998 to serve as the custodian of diagnostic archival formalin fixed paraffin embedded tissues and corresponding hematoxylin and eosin stained slides. Since its creation, specimens have been organized and policies have been instituted for the use of diagnostic archival tissues in research, clinical trials, case study and teaching primarily by OSU investigators/physicians/residents and fellows as well as routine requests for copies of the diagnostic slides by attorneys. Research requests require IRB approval.
The TAS collection of searchable cases dates back to January 1968. The estimated total number of diagnostic tissue blocks in this collection is 11,216,870; the estimated total number of diagnostic slides is 16,825,305.
The Virtual Microscopy Lab (VML) provides a variety of services to researchers and their collaborators locally and around the world to ensure research and education are conducted faster, better, and at lower costs.
The VML provides affordable, high-quality, high-quality digital imaging of tissue sections using the Aperio Scanscope XT scanner by Leica. Slides can be scanned at 5X, 20X, and 40X with the ability to scan standard 1" x 3" slides as well as 2" x 3" slides. A single slide takes about 6 minutes to scan. Scanned image files are in the svs format but can be converted to other formats if needed. Scanned image file size varies but 250 MB is average. Image files can be viewed using Aperio's ImageScope software or ePathViewer for tablets.
Image files hosted on the OSUWMC server can be accessed worldwide using Aperio's eSlide Manager web application. Researchers can view, annotate, and analyze slide images, import and export statistical data, and access pathology reports. eSlide Manager's data elements are fully customizable to fit needed research specifications.