eagle-i The Ohio State UniversityThe Ohio State University

Protein Sample Preparation

eagle-i ID


Resource Type

  1. Material processing service


  1. Fee for service
  2. Resource Description
    Protein Sample Preparation: The PSR accepts cell pellets, tissue, and biological fluids for proteomic analysis, offering all the protein extraction services to properly prepare samples for downstream analysis. This includes: Cell lysis (whole cell or specific cellular) Protein precipitation (TCA and/or chloroform precipitation) Protein/peptide quantitation using standard Bradford, fluorescence assays and NanoDrop Protein cleanup (ziptip desalting) S-trap digestion for proteins in high concentration of SDS or Urea. Protein complex mixture separation by 1D SDS-PAGE (separate complex protein mixture by molecular weight) and commassie blue staining Peptide enrichment such as phosphopeptide enrichment Protein depletion to remove abundant proteins in certain samples such as serum
  3. Service Fee URL
  4. Contact
    Liwen Zhang
  5. Service Provided by
    The CCC Proteomics Shared Resource (PSR)
  6. Website(s)
  7. Website(s)
  8. Part of Collection
    Campus Chemical Instrument Center
  9. Part of Collection
    The Ohio State University Comprehensive Cancer Center Collection
  10. Part of Collection
    Dr. Vicki Wysocki Ohio Eminent Scholar Collection
Provenance Metadata About This Resource Record

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016