Protein Sample Preparation
eagle-i ID
http://eagle-i.rf.ohio-state.edu/i/00000176-2e9d-43a8-ff38-acfe80000000
Resource Type
Properties
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Fee for service
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Yes
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Resource Description
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Protein Sample Preparation: The PSR accepts cell pellets, tissue, and biological fluids for proteomic analysis, offering all the protein extraction services to properly prepare samples for downstream analysis. This includes:
Cell lysis (whole cell or specific cellular)
Protein precipitation (TCA and/or chloroform precipitation)
Protein/peptide quantitation using standard Bradford, fluorescence assays and NanoDrop
Protein cleanup (ziptip desalting)
S-trap digestion for proteins in high concentration of SDS or Urea. Protein complex mixture separation by 1D SDS-PAGE (separate complex protein mixture by molecular weight) and commassie blue staining
Peptide enrichment such as phosphopeptide enrichment
Protein depletion to remove abundant proteins in certain samples such as serum
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Service Fee URL
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http://www.ccic.ohio-state.edu/MSP-Rates
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Contact
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Liwen Zhang
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Service Provided by
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The CCC Proteomics Shared Resource (PSR)
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Website(s)
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https://cancer.osu.edu/for-cancer-researchers/resources-for-cancer-researchers/shared-resources/proteomics
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Website(s)
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https://www.ccic.osu.edu/msp
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Part of Collection
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Campus Chemical Instrument Center
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Part of Collection
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The Ohio State University Comprehensive Cancer Center Collection
