Protein Sample Preparation

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   Protein Sample Preparation: The PSR accepts cell pellets, tissue, and biological fluids for proteomic analysis, offering all the protein extraction services to properly prepare samples for downstream analysis. This includes: Cell lysis (whole cell or specific cellular) Protein precipitation (TCA and/or chloroform precipitation) Protein/peptide quantitation using standard Bradford, fluorescence assays and NanoDrop Protein cleanup (ziptip desalting) S-trap digestion for proteins in high concentration of SDS or Urea. Protein complex mixture separation by 1D SDS-PAGE (separate complex protein mixture by molecular weight) and commassie blue staining Peptide enrichment such as phosphopeptide enrichment Protein depletion to remove abundant proteins in certain samples such as serum

3. Service Fee URL

   http://www.ccic.ohio-state.edu/MSP-Rates

4. Contact

   Liwen Zhang

5. Service Provided by

   The CCC Proteomics Shared Resource (PSR)

6. Website(s)

   https://cancer.osu.edu/for-cancer-researchers/resources-for-cancer-researchers/shared-resources/proteomics

7. Website(s)

   https://www.ccic.osu.edu/msp

8. Part of Collection

   Campus Chemical Instrument Center

9. Part of Collection

   The Ohio State University Comprehensive Cancer Center Collection

10. Part of Collection

    Dr. Vicki Wysocki Ohio Eminent Scholar Collection