Post-Translational Modification Analyses

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   Post-Translational Modification Analyses: Post-translational modification (PTM) is the chemical modification of a protein after translation. These modifications induce a shift in mass, therefore can be identified using mass spectrometry. Common PTM analysis includes phosphorylation, methylation, acetylation and oxidation. Samples may be enriched prior to digestion and then analyzed on the ultra-high-resolution Orbitrap Fusion or timsTOF PRO using LC-MS/MS to determine the sequence of the peptide and the location of the modification. The PSR has extensive experience working with researchers to identify and characterize customized post-translational modifications, amino acid mutations and peptide crosslinks.

3. Service Fee URL

   https://www.ccic.osu.edu/MSP-Rates

4. Contact

   Liwen Zhang

5. Service Provided by

   The CCC Proteomics Shared Resource (PSR)

6. Website(s)

   https://cancer.osu.edu/for-cancer-researchers/resources-for-cancer-researchers/shared-resources/proteomics

7. Website(s)

   https://www.ccic.osu.edu/msp

8. Part of Collection

   Campus Chemical Instrument Center

9. Part of Collection

   The Ohio State University Comprehensive Cancer Center Collection